Scientists at Korea Institute of Science and Technology discuss research in microchannels
2007 NOV 20 -- New investigation results, 'Continuous low-voltage dc electroporation on a microfluidic chip with polyelectrolytic salt bridges,' are detailed in a study published in Analytical Chemistry. According to a study from Seoul, Korea, "A microfluidic electroporator operating under a continuous low dc voltage (7 to approximately 15 V) is reported. The proposed electroporation microchip exploits the ionic conductivity of polyelectrolytic gel electrodes to precisely control the electric field that is applied to cells without bubble generation in the microchannel." "In this study, pDADMAC (poly diallyldimethylammonium chloride) was used to efficiently apply the electric potential difference to the cells in the microchannels. Impedance analysis showed that the pDADMAC plugs could work as ionic conductors with a conductivity of approximately 16 S m(-1). In accordance with the calculation using CFD-ACE, an input voltage of only 10 V could generate an electric field of 0.9 kV cm(-1) across the microchannel; this meets the requirements for electropermeation. The electropermeation of K562 human chronic leukemia cells was observed in the microchip from 7 V, and the efficiency increased up to 60% upon the application of an input voltage of 15 V with a viability of 80%. An amount of 10(5) cells could be transfected every minute under a constant potential difference," wrote S.K. Kim and colleagues, Korea Institute of Science and Technology. The researchers concluded: "The transfection and expression of DNA plasmids were also successfully demonstrated in the suspension cell line." Kim and colleagues published the results of their research in Analytical Chemistry (Continuous low-voltage dc electroporation on a microfluidic chip with polyelectrolytic salt bridges. Analytical Chemistry, 2007;79(20):7761-6). For additional information, contact S.K. Kim, Nanobio Research Center, Korea Institute of Science and Technology, Seoul 130-650, Korea. The publisher of the journal Analytical Chemistry can be contacted at: American Chemical Society, 1155 16th St., NW, Washington, DC 20036, USA. Keywords: Korea, Seoul, Analytical Chemistry, Microchannels, Microfluids, Nanotechnology. This article was prepared by Science Letter editors from staff and other reports. Copyright 2007, Science Letter via NewsRx.com.
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