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Researchers from La Sapienza University discuss findings in gene therapy
2008 FEB 11 -- According to a study from Rome, Italy, "Common fragile sites (CFSs) are chromosome regions that exhibit gaps and breaks when the cells are exposed to replication stress and to some DNA-binding compounds. In cancer cells, the CFSs are frequently involved in recurrent chromosome rearrangements." "Furthermore, altered expression of associated genes, known or potential oncogenes, and tumor-suppressor genes has often been observed. Seventeen of the 88 listed CFSs have been analyzed at the molecular level, but the basis of their fragility has not been clarified. In the present work, the nine genes TGFB2, IARS2, MARK1, TAFI A, TP53BP2, ADPRT, including a very large gene ESRRG and two microRNA genes, MIRN194-1 and MIRN215, localized in the fragile site FRAM, were investigated by polymerase chain reaction (PCR) for homozygous deletions and by real-time PCR for modification or loss of gene expression in a panel of 19 cancer cell lines. The expression level of five (ESRRG, TGFB2, MIRN194-1, MIRN215, and MARK1) of the nine genes studied presented significant modifications in some of the 19 examined tumor-derived cell lines compared to their normal control tissues," wrote F. Pelliccia and colleagues, La Sapienza University. The researchers concluded: "Because of their function, these genes could have a role in neoplastic transformation." Pelliccia and colleagues published the results of their research in Cancer Genetics and Cytogenetics (Transcriptional profiling of genes at the human common fragile site FRA1H in tumor-derived cell lines. Cancer Genetics and Cytogenetics, 2007;178(2):144-150). For additional information, contact A. Rocchi, Roma La Sapienza University, Dipartimento Genetics & Biology Molecular, P le Aldo Moro 5, I-00185 Rome, Italy. The publisher of the journal Cancer Genetics and Cytogenetics can be contacted at: Elsevier Science Inc., 360 Park Avenue South, New York, NY 10010-1710, USA. Keywords: Italy, Rome, Biotechnology, Cancer, DNA, Gene Therapy, Genetics, Genomics, Oncology, Tumor Suppression, La Sapienza University. This article was prepared by Clinical Oncology Week editors from staff and other reports. Copyright 2008, Clinical Oncology Week via NewsRx.com.
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