Reports from X.W. Wang and colleagues advance knowledge in reactive arthritis
2007 NOV 14 -- According to recent research published in the journal Applied Microbiology and Biotechnology, "The rapid and accurate detection and identification of food-borne pathogenic bacteria is critical for food safety. In this paper, we describe a rapid (<4 h) high-throughput detection and identification system that uses universal polymerase chain reaction (PCR) primers to amplify a variable region of bacterial the 16S rRNA gene, followed by reverse hybridization of the products to species-specific oligonucleotide probes on a chip." "This procedure was successful in discriminating 204 strains of bacteria from pure culture belonging to 13 genera of bacteria. When this method was applied directly to 115 strains of bacteria isolated from foods, 112/115 (97.4%) were correctly identified; two strains were indistinguishable due to weak signal, while one failed to produce a PCR product. The array was used to detect and successfully identify two strains of bacteria from food poisoning outbreak samples, giving results through hybridization that were identical to those obtained by traditional methods. The sensitivity of the microarray assay was 10(2) CFU of bacteria," wrote X.W. Wang and colleagues. The researchers concluded: "Thus, the oligonucleotide microarray is a powerful tool for the detection and identification of pathogens from foods." Wang and colleagues published their study in Applied Microbiology and Biotechnology (Development and application of an oligonucleotide microarray for the detection of food-borne bacterial pathogens. Applied Microbiology and Biotechnology, 2007;76(1):225-233). For additional information, contact J.W. Li, Institute Environmental & Health, 1 Dali Rd., Tianjin 300050, People's Republic of China. The publisher's contact information for the journal Applied Microbiology and Biotechnology is: Springer, 233 Spring Street, New York, NY 10013, USA. Keywords: People's Republic of China, Tianjin, Reactive Arthritis, Rheumatology. This article was prepared by Immunotherapy Weekly editors from staff and other reports. Copyright 2007, Immunotherapy Weekly via NewsRx.com.
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