Studies from University of Helsinki reveal new findings on reactive arthritis genetics
2007 JUL 16 -- New research, "Neutrophils of healthy subjects with a history of reactive arthritis show enhanced responsiveness, as defined by CD11b expression in adherent and non-adherent whole blood cultures," is the subject of a report. According to recent research published in the journal Rheumatology, "To study innate immune responsiveness of HLA-B27 positive subjects recovered from Yersinia-triggered reactive arthritis (B27 + ReA+). Whole blood samples from 15 B27 + ReA+, 15 B27 + ReA-and 15 B27 -ReA-subjects were heparinized, aliquoted and (i) kept at 0 degree C to preserve constitutive cell surface marker status, or (ii) cultured with or without bacterial lipopolysaccharide (LPS) supplement, in adherent and non-adherent conditions at 37 degrees C for 4 h." "Neutrophil surface expression of CD11b, CD14 and CD16 was quantified flow cytometrically, and compared between the subject groups using Jonckheere-Terpstra test. The B27 + ReA+ group showed significantly higher CD11b levels than the B27 -ReA-group on non-adherent neutrophils cultured with LPS as 100 pg/ml (p=0.027), 10 ng/ml (p=0.048) or 1 microg/ml (p=0.024), or on adherent neutrophils without LPS supplement (p=0.040). CD14 and CD16 expression on cultured neutrophils and constitutive expression of all three markers were comparable between the groups," wrote K. Kuuliala and colleagues, University of Helsinki. The researchers concluded: "Enhanced neutrophil reactivity observed may exacerbate innate immune inflammation in HLA-B27 positive ReA patients." Kuuliala and colleagues published their study in Rheumatology (Neutrophils of healthy subjects with a history of reactive arthritis show enhanced responsiveness, as defined by CD11b expression in adherent and non-adherent whole blood cultures. Rheumatology, 2007;46(6):934-7). For additional information, contact K. Kuuliala, University of Helsinki, University of Helsinki, Dept. of Bacteriology and Immunology, Finland. The publisher's contact information for the journal Rheumatology is: Oxford University Press, Great Clarendon St., Oxford OX2 6DP, England. Keywords: Finland, Reactive Arthritis Genetics, Reactive Arthritis, Rheumatology. This article was prepared by Pain & Central Nervous System Week editors from staff and other reports. Copyright 2007, Pain & Central Nervous System Week via NewsRx.com.
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