Reactive Arthritis

Studies from Medizinische Hochschule Hannover, Department of Rheumatology reveal new findings on chlamydia

Reactive Arthritis Library Library Home This article was published in OBGYN & Reproduction Week, which you can subscribe to online.

"We developed and evaluated a direct cytometric approach to visualize in vitro C. trachomatis-infected monocytes. Infectious elementary bodies (EBs) of C. trachomatis serovar K were labelled by incubation with 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE). Afterwards, human peripheral blood monocytes were cultured with the CFSE-labelled EBs and analysed by flow cytometry. Real-time polymerase chain reaction (PCR) was used to demonstrate intracellular uptake and viability of CFSE-labelled C. trachomatis by the determination of gene expression," wrote K. Schnitger and colleagues, Medizinische Hochschule Hannover, Department of Rheumatology.

The researchers concluded: "Labelling EBs with CFSE may become a valuable tool for studying the interaction between C. trachomatis and the host cell."

Schnitger and colleagues published the results of their research in the Journal of Microbiological Methods (Staining of Chlamydia trachomatis elementary bodies: a suitable method for identifying infected human monocytes by flow cytometry. Journal of Microbiological Methods, 2007;69(1):116-21).

For additional information, contact K. Schnitger, Medizinische Hochschule Hannover, Dept. of Rheumatology, Hannover 30625, Germany.

The publisher of the Journal of Microbiological Methods can be contacted at: Elsevier Science BV, PO Box 211, 1000 AE Amsterdam, Netherlands.

Keywords: Germany, Hannover, Adolescent Medicine, Chlamydia Infection, Chlamydia Trachomatis, Gynecology, Infectious Disease, Microbiological Methods, Ophthalmology, Women's Health's.

This article was prepared by OBGYN & Reproduction Week editors from staff and other reports. Copyright 2007, OBGYN & Reproduction Week via NewsRx.com.