Rotavirus Vaccine


Studies from INSERM provide new data on rotavirus



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This article was published in Gastroenterology Week, which you can subscribe to online.
2007 NOV 5 -- "We found that at the tight junctions (TJs) of Caco-2 cell monolayers, rhesus monkey rotavirus (RRV) infection induced the disappearance of occludin. Confocal laser scanning microscopy showed the disappearance of occludin from the cell-cell boundaries without modifying the expression of the other TJ-associated proteins, ZO-1 and ZO-3," scientists in Chatenay-Malabry, France report.

"Western immunoblot analysis of RRV-infected cells showed a significant fall in the levels of the nonphosphorylated form of occludin in both Triton X-100-insoluble and Triton X-100-soluble fractions, without any change in the levels of the phosphorylated form of occludin. Quantitative reverse transcription-PCRs revealed that the level of transcription of the gene that encodes occludin was significantly reduced in RRV-infected cells," wrote I. Beau and colleagues, INSERM.

The researchers concluded: "Treatment of RRV-infected cells with Rp-cyclic AMP and protein kinase A inhibitors H89 and KT5720 during the time course of the infection restored the distribution of occludin and a normal level of transcription of the gene that encodes occludin."

Beau and colleagues published their study in the Journal of Virology (A protein kinase A-dependent mechanism by which rotavirus affects the distribution and mRNA level of the functional tight junction-associated protein, occludin, in human differentiated intestinal caco-2 cells. Journal of Virology, 2007;81(16):8579-8586).

For additional information, contact A.L. Servin, INSERM, Unit 510, Faculty Pharmacy, F-92296 Chatenay Malabry, France.

The publisher's contact information for the Journal of Virology is: American Society Microbiology, 1752 N St. NW, Washington, DC 20036-2904, USA.

Keywords: France, Chatenay-Malabry, Enzyme Research, Gastroenterology, Immunization, Kinase, Rotavirus Vaccine, Vaccination, Viral, Virology, Virus, INSERM.

This article was prepared by Gastroenterology Week editors from staff and other reports. Copyright 2007, Gastroenterology Week via NewsRx.com.