Semliki Forest Virus
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New life sciences data have been reported by researchers at Institute for Viral Disease Control and Prevention
2009 JUN 15 - (NewsRx.com) -- A report, 'Selection and characterization of packaging cell lines for XJ-160 virus,' is newly published data in Intervirology. According to a study from Beijing, People's Republic of China, "XJ-160 virus is a mosquito-derived Sindbis-like virus isolated in China. Based on its infectious clone (pBR-XJ-160) we have developed an RNA-based vector system." "In this work, we constructed packaging cell lines (PCLs) for XJ-160 virus. Firstly, XJ-160 virus structural protein expression cassette pcE or pICH was constructed by cloning the gene of glycoprotein E3E26KE1 or capsid protein of XJ-160 virus into pcDNA3.1(+) or pIRES, respectively. Then the PCLs (BHK-21(E+Capsid) cells) for XJ-160 virus were obtained by two selections with G418 and hygromycin. The results indicate that BHK-21(E+Capsid) cells, stably expressing E3E26KE1 protein and capsid protein of XJ-160 virus, not only highly increased packaging efficiency of the vector from XJ-160 virus, but also provided packaging function for the vector from Semliki Forest virus. These results suggest potential utility of the PCLs of XJ-160 virus for large-scale vector production and facilitating broad alphavirus applications," wrote W.Y. Zhu and colleagues, Institute for Viral Disease Control and Prevention. The researchers concluded: "Also, the construction of PCLs for XJ-160 virus lays a basis for developing alphavirus-derived vector systems." Zhu and colleagues published the results of their research in Intervirology (Selection and characterization of packaging cell lines for XJ-160 virus. Intervirology, 2009;52(2):100-6). For additional information, contact W.Y. Zhu, State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Yingxin Street, Beijing, China. The publisher of the journal Intervirology can be contacted at: S. Karger AG, Allschwilerstrasse 10, CH-4009 Basel, Switzerland. Keywords: People's Republic of China, Beijing, Life Sciences, Biotechnology, Vector Systems, Gene Therapy, Protein Expression, Proteomics, Proteins, Intervirology, Pathobiology, Virology, Cloning. This article was prepared by Proteomics Weekly editors from staff and other reports. Copyright 2009, Proteomics Weekly via NewsRx.com.
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