Research from University of Oxford has provided new data on DNA research
2009 JUL 27 - (NewsRx.com) -- "The RAD51 paralogues act in the homologous recombination (HR) pathway of DNA repair. Human RAD51C (hRAD51C) participates in branch migration and Holliday junction resolution and thus is important for processing HR intermediates late in the DNA repair process," scientists in Oxford, the United Kingdom report. "Evidence for early involvement of RAD51 during DNA repair also exists, but its function in this context is not understood. In this study, we demonstrate that RAD51C accumulates at DNA damage sites concomitantly with the RAD51 recombinase and is retained after RAD51 disassembly, which is consistent with both an early and a late function for RAD51C. RAD51C recruitment depends on ataxia telangiectasia mutated, NBS1, and replication protein A, indicating it functions after DNA end resection but before RAD51 assembly. Furthermore, we find that1 RAD51C is required for activation of the checkpoint kinase CHK2 and cell cycle arrest in response to DNA damage," wrote S. Badie and colleagues, University of Oxford. The researchers concluded: "This suggests that hRAD51C contributes to the protection of genome integrity by transducing DNA damage signals in addition to engaging the HR machinery.." Badie and colleagues published their study in the Journal of Cell Biology (RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation. Journal of Cell Biology, 2009;185(4):587-600). For more information, contact M. Tarsounas, University of Oxford, Gray Institute Radiation Oncology & Biology, Cancer Research UK Med Research Council, Oxford OX3 7DQ, UK. Publisher contact information for the Journal of Cell Biology is: Rockefeller University Press, 1114 First Avenue, 4TH FL, New York, NY 10021, USA. Keywords: United Kingdom, Oxford, Cell Biology, DNA Damage, DNA Repair, DNA Research, Deoxyribonucleic Acid, Genetics, Proteomics, University of Oxford. This article was prepared by Proteomics Weekly editors from staff and other reports. Copyright 2009, Proteomics Weekly via NewsRx.com.
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