Teratoma

Reports outline stem cell research study results from German Primate Center

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"The common marmoset monkey (Callithrix jacchus) is a useful preclinical non-human primate model due to its physiological similarities to human. Yet, few marmoset ESC lines exist and differences in their developmental potential remain unclear. Blastocysts were collected and immunosurgery was performed. cjes001 cells were tested for euploidy by karyotyping. The presence of markers for pluripotency was confirmed by immunofluorescence staining and RT-PCR. Histology of teratoma, in vitro differentiation and embryoid body formation revealed the differentiation potential. cjes001 cells displayed a normal 46,XX karyotype. Alkaline phosphatase activity, expression of telomerase and the transcription factors OCT4, NANOG and SOX2 as well as the presence of stage-specific embryonic antigen (SSEA)-3, SSEA-4, tumor rejection antigens (TRA)-1-60, and TRA-1-81 indicated pluripotency. Teratoma formation assay displayed derivatives of all three embryonic germ layers. Upon non-directed differentiation, the cells expressed the germ cell markers VASA, BOULE, germ cell nuclear factor and synaptonemal complex protein 3 and showed co-localization of VASA protein within individual cells with the germ line stem cell markers CD9, CD49f, SSEA-4 and protein gene product 9.5, respectively. The cjes001 cells represent a new pluripotent ESC line with evidence for enhanced spontaneous differentiation potential into germ cells," wrote T. Muller and colleagues, German Primate Center.

The researchers concluded: "This cjes001 line will be very valuable for comparative studies on primate ESC biology."

Muller and colleagues published their study in Human Reproduction (A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics. Human Reproduction, 2009;24(6):1359-1372).

For additional information, contact R. Behr, German Primate Center, Stem Cell Research Group, Kellnerweg 4, D-37077 Gottingen, Germany.

Publisher contact information for the journal Human Reproduction is: Oxford University Press, Great Clarendon St., Oxford OX2 6DP, England.

Keywords: Germany, Clinical Trials, Diagnosis, Diagnostics, Drug Development, Embryonic Stem Cell Research, Histology, Pharmaceuticals, Physiology, Pre-Trials Research, Surgery, Teratoma, Therapy, Treatment, Urology, German Primate Center.

This article was prepared by Biotech Business Week editors from staff and other reports. Copyright 2009, Biotech Business Week via NewsRx.com.