Studies from University of Gottingen update current data on immunofluorescence
2009 JUL 20 - (NewsRx.com) -- According to recent research from Germany, "The tumor suppressor homologue p63 is required for proper skin and limb development, but specific isoforms of it also act as a ''guardian of the germline.'' To gain insight into the regulation of p63 expression, we performed immunofluorescence-based screening assays. Using a large collection of microRNA expression plasmids, we identified microRNAs of the 302 cluster as potent suppressors of p63 accumulation in various cell species." "MiR-302 reduces p63 protein and mRNA levels through two target sites within the p63 3' untranslated region. In testicular cancer cells, endogenous miR-302 contributes to the suppression of p63. MiR-302 might also contribute to the elimination of p63 in mature oocytes," wrote A.H.J. Scheel and colleagues, University of Gottingen. The researchers concluded: "Thus, miR-302 appears as part of a stringent regulatory mechanism for p63 in germ cells, reminiscent of the tight control for p53 levels in somatic cells." Scheel and colleagues published their study in Cell Cycle (Immunofluorescence-based screening identifies germ cell associated microRNA 302 as an antagonist to p63 expression. Cell Cycle, 2009;8(9):1426-1432). For additional information, contact M. Dobbelstein, University of Gottingen, Dept. of Molecular Oncology, Gottingen Center Molecular Bioscience, Justus von Liebig Eg 11, D-37077 Gottingen, Germany. Publisher contact information for the journal Cell Cycle is: Landes Bioscience, 1002 West Avenue, 2ND Floor, Austin, TX 78701, USA. Keywords: Germany, Immunofluorescence, Diagnosis, Diagnostics, Oncology, Testicular Cancer, Testicular Diseases, Tumor Suppression, University of Gottingen. This article was prepared by Clinical Oncology Week editors from staff and other reports. Copyright 2009, Clinical Oncology Week via NewsRx.com.
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