New DNA research findings from New England Medical Center, Department of Pediatrics published
2007 AUG 20 -- Research findings, "Array-CGH analysis of cell-free fetal DNA in 10 mL of amniotic fluid supernatant," are discussed in a new report. "Previously, we showed that analysis of amniotic fluid (AF) supernatant cell-free fetal (cff) DNA using DNA microarrays (array-CGH) allows for detection of whole chromosome differences between test and reference DNA. Subsequent technical advances have increased both the yield and quality of extracted cffDNA," scientists writing in the journal Prenatal Diagnosis report. "Here we determined whether array-CGH using smaller volumes of both fresh and frozen AF cffDNA could identify fetal aneuploidy. CffDNA was extracted from 10 mL of residual AF supernatant. The test AF samples (n=10) included one with a normal karyotype, and nine with the following fetal aneuploidies: trisomies 13 (n=1), 18 (n=3), 21 (n=2), trisomy 9 mosaicism (47,XX,+ 9[18]/46,XX[2]), triploidy (69,XXY) and Turner syndrome (45,X). Array-CGH using AF cffDNA from aneuploid fetuses, compared to euploid reference AF cffDNA, detected whole chromosome aneuploidy in 8 of 9 cases tested, including the case of trisomy 9 mosaicism. The case of triploidy was not detected. CffDNA extracted from 10 mL AF supernatant can be analyzed using array-CGH to correctly identify human chromosome abnormalities," wrote O. Lapaire and colleagues, New England Medical Center, Department of Pediatrics. The researchers concluded: "This technology allows for rapid screening of AF samples for whole chromosomal changes by using routinely discarded supernatant, and may augment standard prenatal karyotyping techniques by providing additional molecular information." Lapaire and colleagues published their study in Prenatal Diagnosis (Array-CGH analysis of cell-free fetal DNA in 10 mL of amniotic fluid supernatant. Prenatal Diagnosis, 2007;27(7):616-21). Additional information can be obtained by contacting O. Lapaire, Tufts-New England Medical Center, Dept. of Pediatrics, Division of Genetics, Boston, MA USA.. The publisher of the journal Prenatal Diagnosis can be contacted at: John Wiley & Sons Ltd., the Atrium, Southern Gate, Chichester PO19 8SQ, W Sussex, England. Keywords: United States, Boston, DNA Research, DNA. This article was prepared by Proteomics Weekly editors from staff and other reports. Copyright 2007, Proteomics Weekly via NewsRx.com.
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