Diagnostics

HIV-1 RNA Isolated in Feces Using Simple Method

Published in AIDS Weekly, April 10th, 1995

There may be a link between the route of HIV-1 transmission and the presence of HIV-1 in the intestinal lumen and between the onset of gastrointestinal symptoms and the detection of HIV-1 RNA in feces, according to a report from the Netherlands.

Purification of viral RNA from feces is difficult by the most common extraction methods, presumably because of its extremely varying composition. In earlier studies researcher L. Van Der Hoek and colleagues reported on a reliable and rapid procedure for the purification of nucleic acids from clinical specimens like serum and urine. This procedure is based on the lysing and nuclease-inactivating properties of the chaotropic...

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Source: AIDS Weekly (1995-04-10)

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