"Detection and Identification of Mycobacteria by DNA Amplification and Oligonucleotide-Specific Capture Plate Hybridization."

According to the authors' abstract of an article published in Journal of Clinical Microbiology, "We have developed an easy and rapid detection and identification system for the diagnosis of mycobacterial diseases. The system is based on selective amplification by PCR of mycobacteria with primers based on the genes coding for 16S rRNA. During PCR, a label (digoxigenin-11-dUTP) is incorporated in the amplicon. After amplification, the amplicon is hybridized in streptavidin-coated microtiter plates prepared with biotinylated species-specific oligonucleotides (oligonucleotide-specific capture plate hybridization [OSCPH]). One oligonucleotide specific for the genus Mycobacterium and...

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