"HIV Infection in the Bone Marrow: Detection by PCR Amplified In Situ Hybridization."

erican Society of Hematology, held December 1-5, 1995, in Seattle, Washington, "Hematological abnormalities are a frequent finding in HIV disease. In animals, the bone marrow serves as a reservoir for lentiviruses, the family of viruses that includes HIV-1. However, the incidence and extent of bone marrow infection with HIV-1 in vivo is not known. Using in-situ hybridization (ISH) for viral RNA, we have previously shown that only 16% of bone marrow biopsies of patients with end-stage AIDS demonstrated active viral infection. However, these studies could not exclude proviral DNA or low level virus present in the marrow below the detection of standard ISH. To overcome this difficulty,...

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