"Detection of HIV-1 RNA by Nucleic Acid Sequence-Based Amplification Combined with Fluorescence Correlation Spectroscopy."

According to the authors' abstract of an article published in Proceedings of the National Academy of Sciences of the United States of America, "Nucleic acid sequence-based amplification (NASBA) has proved to be an ultrasensitive method for HIV-1 diagnosis in plasma even in the primary HIV infection stage. This technique was combined with fluorescence correlation spectroscopy (FCS) which enables online detection of the HIV-1 RNA molecules amplified by NASBA. A fluorescently labeled DNA probe at nanomolar concentration was introduced into the NASBA reaction mixture and hybridizing to a distinct sequence of the amplified RNA molecule. The specific hybridization and extension of this probe...

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