"Stable Human Immunodeficiency Virus Type 1 (HIV-1) Resistance in Transformed CD4⁺ Monocytic Cells Treated with Multitargeting HIV-1 Antisense Sequences Incorporated int

According to the authors' abstract of an article published in Journal of Virology, "We have approached the development of a human immunodeficiency virus type 1 (HIV-1) therapeutic product by producing immune cells stably resistant to HIV-1. Promonocytic CD4⁺ cells (U937) were made resistant to HIV-1 by the introduction of a DNA construct (pNDU1A,B,C) that contained three independent antisense sequences directed against two functional regions, transactivation response and tat/rev, of the HIV-1 target. Each sequence was incorporated into the transcribed region of a U1 snRNA gene to generate U1/HIV antisense RNA. Stably transfected cells expressed all three U1/HIV antisense...

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