"Reverse Transcriptase Inhibitors Can Selectively Block the Synthesis of Differently Sized Viral DNA Transcripts in Cells Acutely Infected With Human Immunodeficiency Virus Type 1."

According to the authors' abstract of an article published in Journal of Virology, "We have recently reported that the in vitro inhibition of human immunodeficiency virus type 1 (HIV-1) reverse transcription by inhibitors of reverse transcriptase (RT) occurred most efficiently when the expected DNA products of RT reactions were long (Quan et al., Nucleic Acids Res. 1998;26:5692-5698). Here, we have used a quantitative PCR to analyze HIV-1 reverse transcription within acutely infected cells treated with RT inhibitors. We found that levels of minus-strand strong-stop DNA [^-ssDNA] formed in acutely infected Mn cells were only slightly reduced if cells were infected with viruses...

Want to see the full article?

• Purchase this article for only $3.00