Published in AIDS Weekly, January 5th, 2004
In a recent study from Switzerland, "An internally controlled high-input PCR method, termed HIV-1 Mega-PCR was developed to lower the detection limit of HIV-1 DNA polymerase chain reaction (PCR) and to improve its value as a complementary diagnostic test."
This assay "is based on PCR amplification of two target sequences in the gag gene of HIV-1 following the selective capture of the targeted sequence and removal of unselected DNA from up to 500 microg of DNA," explained J. Boni and coauthors at the University of Zurich.
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