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AIDS/HIV Vaccine

HIV cholera toxin fusion gene expressed in transgenic potato

Published in AIDS Weekly, October 25th, 2004

Scientists have achieved HIV-1 gp120 V3 cholera toxin B subunit fusion gene expression in a transgenic potato.

"A cDNA fragment encoding the V3 loop of human immunodeficiency virus type-1 (HIV-1) envelope glycoprotein gp120 was fused to the cholera toxin B subunit gene (CTB-gp120) and transferred into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation. The CTB-gp 120 fusion gene was detected in genomic DNA from transformed potato leaves by PCR DNA amplification. Synthesis and assembly of the CTB-gp120 fusion protein into oligomeric structures of pentamer size was detected in transformed tuber extracts by immunoblot analysis,"...

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