HIV/AIDS Research

Lentiviral cDNA library used to clone HIV-1-induced cell death inhibitor

Published in AIDS Weekly, December 27th, 2004

A lentiviral cDNA library was used to clone an HIV-1-induced cell death inhibitor.

According to recent research published in the Journal of Virology, "Expression cloning technology of cDNAs is a suitable tool for identifying novel functional properties of genes.

"Here, we generated a lentiviral cDNA library-expressing system for human T cells based on a site-specific recombination system of phage lambda for transferring cDNA libraries with a minimum loss of its complexity."

"The library-transduced CD4+ T cells were challenged with wild-type human immunodeficiency virus type 1 (HIV-1), and the cells that acquired resistance...

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Source: AIDS Weekly (2004-12-27)

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