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HPV-16 DNA from genital specimens is comparable in three real-time PCR assays

Published in AIDS Weekly, November 21st, 2005

Human papillomavirus-16 DNA from genital specimens is comparable in three real-time PCR assays.

"Human papillomavirus-16 (HPV-16) viral load could be a biomarker predictive of the presence of high-grade cervical lesions. Recently, several real-time PCR assays have been developed to accurately measure HPV-16 viral load.

"However, results from various reports using these assays cannot be compared because interassay test correlation has not been documented," scientists in Canada report.

"The variability of HPV-16 DNA quantitation was assessed by comparing three real-time PCR assays (HPV-16 L1, HPV-16 E6, and HPV-16 E6 PG) applied on...

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