HIV/AIDS

New HIV/AIDS research from Keio University outlined

Published in AIDS Weekly, September 3rd, 2007

"An easy, inexpensive competitive RT-PCR assay for HIV-1 RNA quantitation was constructed. A 138-bp sequence in the HIV-1 gag p24 region was selected as the target and co-amplified with competitor RNA containing an internal 44-bp deletion," scientists writing in the Journal of Virological Methods report.

"Quantitation of serial dilutions of control RNA samples prepared from the LAI isolate demonstrated a good linearity (R-2 = 0.991) within the range between 10 and 250 copies/sample. The detection limit of the assay was determined to be 3.8copies/sample by Probit analysis and corresponded to 110copies/ml in plasma. The intraassay CV value was 9.1%, and the...

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Source: AIDS Weekly (2007-09-03)

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