HIV/AIDS

New HIV/AIDS research reported from University of Quebec

Published in AIDS Weekly, March 10th, 2008

"We and others have recently demonstrated that the human retrovirus HTLV-1 was producing a spliced antisense transcript, which led to the synthesis of the HBZ protein. The objective of the present study was to demonstrate the existence of antisense transcription in HIV-1 and to provide a better characterization of the transcript and its regulation," investigators in Montreal, Canada report.

"Initial experiments conducted by standard RT-PCR analysis in latently infected J1.1 cell line and pNL4.3-transfected 293T cells confirmed the existence of antisense transcription in HIV-1. A more adapted RT-PCR protocol with limited RT-PCR artefacts also led to a successful...

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Source: AIDS Weekly (2008-03-10)

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