HIV/AIDS

Researchers from New York University, Medical Department report details of new studies and findings in the area of HIV/AIDS

Published in AIDS Weekly, February 16th, 2009

Researchers in the United States conducted a study "To determine the frequency of dual inter- and intra-subtype HIV-1 infection among a cohort of 64 longitudinally-studied, HIV-1-positive individuals in Yaounde, Cameroon. Blood was collected every 3-6 months for up to 36 months and RNA was extracted from plasma."

"Gag fragment (HxB2 location 1577-2040) was amplified by nested RT-PCR, and mixed-time-point Heteroduplex Assays (HDAs) were performed. As heteroduplexes in this assay indicate >= 5% genetic discordance in the gag fragment, their presence reveals dual infection. Results were confirmed by phylogenetic analysis. Heteroduplexes were generated by specimens of 10...

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Source: AIDS Weekly (2009-02-16)

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