Diagnostics
Nested PCR more sensitive than real-time PCR for detection of T. gondii
Published in Anti-Infectives Week, September 20th, 2004
"Toxoplasma reactivation is a serious complication in patients receiving allogenic stem cell transplantation. Real-time PCR assays allow a rapid diagnosis of toxoplasma infection; however, no comparative data are available on the performance of real-time PCR protocols under routine conditions.
"Therefore, the aim of this study was to amplify Toxoplasma gondii DNA from routine samples of allogenic stem cell recipients using two real-time PCR assays on a LightCycler, and using conventional nested PCR," researchers in Germany report.
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Source: Anti-Infectives Week (2004-09-20)
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