Respiratory Syncytial Virus

Bovine respiratory syncytial virus detected/quantitated by real-time RT-PCR

Published in Anti-Infectives Week, December 13th, 2004

Bovine respiratory syncytial virus was detected and quantitated by real-time RT-PCR.

"A single tube, fluorogenic probe-based, real-time quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay was developed for detection and quantitation of bovine respiratory syncytial virus (BRSV) using BioRad's iCycler iQ.

"Real-time Q-RT-PCR was compared with quantitative competitive RT-PCR (QC-RT-PCR) and viral titers. Viral mRNA levels were measured in BRSV-infected bovine turbinate cell lysate harvested at eight time points (1.5, 6, 12, 24, 36, 48, 60, 72 hours) post-infection," scientists in the United States report.

"A...

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Source: Anti-Infectives Week (2004-12-13)

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