Cryptococcus Diagnostics

RT-PCR assay detects and quantitates Cryptococcus neoformans

Published in Anti-Infectives Week, March 21st, 2005

A real-time-PCR assay is used to detect and quantitate Cryptococcus neoformans.

"Cryptococcus neoformans is an opportunistic fungal pathogen. It infects the central nervous system causing meningitis, which is fatal if untreated, especially in AIDS and immunosuppressed patients.

"In this study a method of quantification and assessment of viability of C. neoformans by LightCycler RTPCR amplification of the capsule gene mRNA is established," scientists writing in the Journal of Medical Microbiology report.

"The sequence of primers and probes were derived from C. neoformans capsular CAP10 gene mRNA...

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Source: Anti-Infectives Week (2005-03-21)

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