Published in Biotech Business Week, January 26th, 2004
According to a study from England, "A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative a-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)6 at the C-terminus, and its purification in milligram quantities."
"The retention of structural and functional integrity was verified by circular dichroism spectroscopy and...
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