Biotech Law Weekly
Welcome to NewsRx!
Learn more about a six-week, no-risk free trial of Biotech Law Weekly
We're a pay-per-view site for premium content. If you'd like to purchase this article, it's only $3.00.
Argonne National Laboratory, U.S.
Vector for large-scale, high-throughput purification of recombinant proteins has been developed
August 18th, 2006
Scientists have developed an expression vector tailored for large-scale, high-throughput purification of recombinant proteins. According to recent research from the United States, "Production of milligram quantities of numerous proteins for structural and functional studies requires an efficient purification pipeline. We found that the dual tag, his6 -tag-maltose-binding protein (MBP), intended to facilitate purification and enhance proteins' solubility, disrupted such a pipeline, requiring additional screening and purification steps." "Not all proteins rendered soluble by fusion to MBP remained soluble after its proteolytic removal, and...
Source: Biotech Law Weekly (2006-08-18)
|
