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Diagnostics - New Marker of HCV Infection

"Antibody to Hepatitis C Virus Second Envelope (HCV-E2) Glycoprotein: A New Marker of HCV Infection Closely Associated with Viremia."

Published in Blood Weekly, May 15th, 1995

According to the authors' abstract of an article published in Journal of Medical Virology, "The second envelope protein (E2) of the hepatitis C virus (HCV) was cloned and expressed in Chinese hamster ovary (CHO) cells. This E2 glycoprotein was purified using ion exchange and lectin chromatography and used to construct an enzyme immunoassay for HCV E2 antibodies. The assay was shown to have good specificity, and detection of E2 antibodies was positively correlated (97.3%) to the presence of HCV RNA in serum and plasma. A high concordance between HCV 2.0 and E2 EIA reactivities was also observed. E2 antibody was the first serological marker to appear in 3/5 HCV seroconversion panels. ...

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