"Gene Cloning and Purification of Proteins Secreted by M. tuberculosis."

According to an abstract submitted by the authors to the 96th General Meeting of the American Society for Microbiology, held May 19-23, 1996, in New Orleans, Louisiana, "The long-term goal of our studies is to identify protein antigens actively secreted by Mycobacterium tuberculosis that elicit protective T cell responses against TB and/or stimulate humoral immunity. Such antigens can be valuable for production of subunit vaccines and for development of serological diagnosis of TB. We have isolated genes expressing M. tuberculosis secreted proteins using two different approaches: 1) by PCR-based methods using published nucleotide sequences for primer design, and 2) by...

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