"A Sensitive Sandwich ELISA for Measuring Thrombopoietin in Human Serum: Serum Thrombopoietin Levels in Healthy Volunteers and in Patients with Haemopoietic Disorders."

According to the authors' abstract of an article published in British Journal of Haematology, "A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) has been established to estimate serum thrombopoietin (TPO) concentrations in healthy volunteers and patients with haemopoietic disorders. The ELISA uses a mouse monoclonal antibody (Ab) as the capture Ab and a biotinylated rabbit polyclonal Ab as the detector. The ELISA was reproducible, highly sensitive and specific for human TPO. The coefficients of intra- and interassay variation were from 3.0% to 4.9% and from 5.9% to 6.1%, respectively. The quantitative limit of the ELISA was 0.09 fmol/ml in serum. The quantitative limit...

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