"Comparison of a Competitive Combined Reverse Transcription-PCR Assay with a Branched-DNA Assay for Hepatitis C Virus RNA Quantitation."

According to the authors' abstract of an article published in Journal of Clinical Microbiology, "We have developed a sensitive and reproducible one-step competitive reverse transcriptase (RT) PCR assay, which allows hepatitis C virus (HCV) RNA quantitation in plasma over a broad range of values. The RNA samples and a constant amount of an internal standard were reverse transcribed and coamplified with the same primers in the same tube. A standard curve was obtained from an additional series of tubes containing both the internal standard and known amounts of a wild-type HCV RNA transcript, thus eliminating the need for titrating samples with the competitor. Eighty-eight anti-HCV-positive...

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