"Microdroplet Fluorochromatic Assay for the Enumeration of White Cells (WBCs) in WBC-Reduced Blood Components: Validation and Application for Evaluating Newly Developed WBC-Reduction Filters.

According to the authors' abstract of an article published in Transfusion, "Background: Sensitive and accurate counting methods are required to assess the residual white cells (WBCs) in WBC-reduced blood components. The Nageotte hemocytometer, widely used for this purpose, is cumbersome, and its efficacy is dependent upon the skill of the operator. The performance of a simple fluorochromatic assay using tissue-typing microdroplet trays is presented here. Study Design and Methods: Undiluted samples of blood components were mixed with a fluorochromatic dye in trays. WBCs were counted under an epifluorescence microscope. The accuracy and sensitivity of this method were...

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