Transplantation
Gene Gun DNA Transfer Useful for Graft Manipulation
Published in Blood Weekly, August 11th, 1997
The genetic manipulation of grafts, in particular cellular grafts such as islets, before transplantation represents a therapeutic approach for enhancing islet graft survival. Although ex vivo gene therapy techniques have received recent attention, this method is limited by the efficiency of gene transfer techniques.
Islet cells are organized like a 'miniorgan' and do not replicate. As a result, the standard techniques such as cationic liposome-mediated transfection, calcium phosphate co-precipitation, or retroviral infection are...
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Source: Blood Weekly (1997-08-11)
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