"Quantification of HIV-1 Viral RNA and Proviral DNA by Isotopic Competitive PCR."

According to the authors' abstract of an article published in Journal of Virological Methods, "A quantitative isotopic competitive PCR (icPCR) assay was established using P-32-labeled primers targeting the HIV-1 gag gene followed by quantification using a phosphoimager. The detection limit varied from 3 to 10 molecules of DNA and 10 to 100 molecules of RNA per reaction. The icPCR quantification of HIV-1 DNA copies correlated well with the cell number of 8E5/LAV cells bearing a single provirus (r(2) = 0.95). Provirus quantification was applied to overnight infected donor PBMCs, thereby determining infectious virus titres in culture supernatants as a rapid alternative to limiting dilution...

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