Diagnostics

Lysis Method Identifies Mycobacteria in Blood and Bone Marrow

Published in Blood Weekly, September 8th, 1997

A sodium dodecyl sulfate method allows the recovery of mycobacteria from blood specimens and bone marrow aspirates, according to a report from Spain.

DNA amplification by polymerase chain reaction (PCR) has the potential to reduce the time taken for the direct identification of Mycobacterium tuberculosis complex and M. avium-M. intracellulare complex (MAC) by allowing the use of diverse genetic elements as target sequences. Problems still exist, however, including the presence of inhibitors of the PCR which may cause false-negative results (Clarridge et al., J Clin Microbiol 1993;31:2049-2056).

The isolation of small amounts of DNA...

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Source: Blood Weekly (1997-09-08)

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