"A Whole Blood Assay to Detect T Cell Response to Antigens in HIV-1 Infected Patients."

According to an abstract submitted by the authors to the 8th European Congress of Clinical Microbiology and Infectious Diseases, held May 25-28, 1997, in Lausanne, Switzerland, "Objectives: We developed an heparinized whole blood (HWB) assay to measure T cell mediated immune responses to HIV and recall antigens. Methods: The cultures were performed in 96-well plates in which 25 (mu)l of whole blood was required. We studied the production of gamma and IL-4 in short term culture (24 and 48 h) of 1/10 diluted HWB cultures from 15 healthy controls and 43 HIV-1 infected patients grouped according to 1993 classification of the CDC. IFN-gamma and IL-4 were measured with an...

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