"Amino Acids Responsible for Decreased 2,3-Biphosphosphoglycerate Binding to Fetal Hemoglobin."

According to the authors' abstract of an article published in Blood, "To clarify the role of gamma N-terminal Gly, gamma 5 Glu, and gamma 143 Ser in 2, 3-biphosphosphoglycerate (BPG) binding to fetal hemoglobin (Hb F), we engineered and produced normal human Hb F and two Hb F variants (Hb F gamma G1V, gamma S143H, and Hb F gamma G1V, gamma E5P, gamma S143H) using a yeast expression system and then compared their oxygen-binding properties with those of native human Hb F and adult Hb (Hb A). Oxygen affinity of Hb F gamma G1V, gamma S143H in the absence of 2, 3-BPG was slightly higher than that of normal Hb F. The decrease in oxygen affinities for Hb F gamma G1V, gamma S143H with increasing...

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