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CMV

FACS-Based Method Isolates and Quantifies Cytomegalic Endothelial Cells

Published in Blood Weekly, November 23rd, 1998

A fluorescence-activated cell sorting method to quantify cytomegalic endothelial cells in peripheral blood from patients with cytomegalovirus infection has several advantages, researchers reported.

A.M. Kas-Deelen and colleagues, University Hospital Groningen, Netherlands, developed the sensitive fluorescence-activated cell sorting (FACS) method ("A Sensitive Method for Quantifying Cytomegalic Endothelial Cells in Peripheral Blood from Cytomegalovirus-Infected Patients," Clinical and Diagnostic Laboratory Immunology, 1998;5:622-626).

The three subsequent major steps of the method are density centrifugation to enrich endothelial cells (EC) in the...

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