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Vector Development

Electroporation Transfects Peripheral Blood Lymphocytes

Published in Blood Weekly, February 3rd, 2000

by Michelle Marble -- Human peripheral blood lymphocytes (PBL) were successfully transfected with electroporation, reported researchers from the United Kingdom.

"To explore the feasibility of ex vivo lymphocyte gene therapy, we evaluated transient transfection of primary cultures of human PBLs by the non-viral method of electroporation," wrote S. Islam and O. Eremin from the University of Aberdeen, United Kingdom.

The researchers performed proliferation studies to optimize conditions for the long-term culture and expansion of PBLs using T-cell mitogens (PHA, IL-2, Con A). Results from their study were presented at the Joint Congress of the British...

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