Published in Blood Weekly, February 3rd, 2000
"To explore the feasibility of ex vivo lymphocyte gene therapy, we evaluated transient transfection of primary cultures of human PBLs by the non-viral method of electroporation," wrote S. Islam and O. Eremin from the University of Aberdeen, United Kingdom.
The researchers performed proliferation studies to optimize conditions for the long-term culture and expansion of PBLs using T-cell mitogens (PHA, IL-2, Con A). Results from their study were presented at the Joint Congress of the British...
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