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Ultrasensitive Test Measures HIV-1 RNA in Plasma

Published in Blood Weekly, August 10th, 2000

Scientists in Italy have updated an HIV-1 RNA detection assay.

"An ultrasensitive version of an 'in-house' reverse transcription-competitive polymerase chain reaction assay described previously for quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma was developed," wrote G. Venturi and colleagues, University of Siena, Italy. "The increase in sensitivity from 400 to 50 HIV-1 RNA copies/ml was achieved by pelleting virus particles from 1.8 ml plasma by centrifugation prior to RNA extraction, modifying competitor DNA structure and amounts, and redesigning primers."

Venturi et al. described their assay in the Journal of...

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