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Stem Cell Research

Combination Counting Method Provides Increased Accuracy

Published in Blood Weekly, November 1st, 2001

by Michael Greer, senior medical writer - Researchers in Australia say that combining two techniques for counting stem cells in a single platform results in more accurate quantification of these cells.

"Single-platform flow cytometric absolute cell counting protocols provide increased robustness for CD34+ cell enumeration by limiting potential sources of imprecision," according to Rosemary L. Sparrow and colleagues at the Australian Red Cross Blood Service in Victoria. "However, samples with any cellular fragmentation or debris, such as card blood samples, provide challenges for these assays."

Combining robust counting methods with gating...

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