Gene Expression

Method achieves RNA interference in differentiated embryonic stem cells

Published in Blood Weekly, June 5th, 2003

According to University of Cambridge geneticists, "dsRNA of several hundred nucleotides in length is effective at interfering with gene expression in mouse oocytes, pre-implantation embryos, and embryonic stem (ES) cells, but is not as efficient in differentiated cell lines."

So the researchers set about developing a way to obtain more efficient RNA interference in differentiated cells and came up with a technique that works "in totipotent and differentiated ES cells together with a wide range of other mammalian cell types that is both simple and efficient," said J.B. Grabarek and colleagues.

A linearized plasmid is used "that directs the expression...

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Source: Blood Weekly (2003-06-05)

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