Stem-Cell Research
Advantages of 3D stem-cell coculture system clarified
Published in Blood Weekly, November 13th, 2003
Scientists in Japan "previously reported that a 3D coculture system for hematopoietic cells with spatial development of stromal cells in nonwoven fabrics was superior to a conventional 2D coculture system with respect to progenitor expansion and stem cell maintenance."
In a subsequent study, "the hematopoietic environments in 2D and 3D coculture systems were compared," according to T. Sasaki and coauthors at Osaka University.
They found "no differences in the amounts of soluble factors, e.g., mRNA levels of stem cell factor (SCF) and thrombopoietin (TPO), the...
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Source: Blood Weekly (2003-11-13)
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