Stem-Cell Research

Cost-effective method for producing chimeric animals from ES cells developed

Published in Blood Weekly, January 8th, 2004

A cost-effective technique has been developed for generating chimeric animals from ES cells.

According to published research from Philadelphia, "Gene targeting in embryonic stem (ES) cells followed by preparation of chimeric animals is the most effective method to study the function of a gene during development and differentiation."

P.U. Rani and coauthors working at the Wistar Institute of Anatomy and Biology developed "a cost-effective and convenient method to produce chimeric animals."

"Cryopreserved 8-16 cell mouse embryos were aggregated with ES cells in microwell petridishes (Khillan & Bao, 1997) to obtain blastocysts," they...

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Source: Blood Weekly (2004-01-08)

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