Malaria Therapy
Novel LC assay accurately measures AQ and AQm in whole blood
Published in Blood Weekly, February 19th, 2004
Scientists in Kenya "developed a sensitive, selective and reproducible reversed-phase HPLC method with ultraviolet detection (340 nm) for the simultaneous quantification of amodiaquine (AQ) and its major metabolite, desethylamodiaquine (AQm) in a small volume (200 microL) of whole blood spotted on filter paper."
"The method involves liquid-liquid extraction with diethyl ether followed by elution from a reversed-phase phenyl column with an acidic (pH 2.8) mobile phase (25 mM KH2PO4-methanol; 80:20%...
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Source: Blood Weekly (2004-02-19)
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