Retrovirology
Luciferase-based assay rapid and sensitive for detection of retrovirus entry
Published in Blood Weekly, July 22nd, 2004
According to published research from the United States, "We describe a novel assay that permits measurement of entry of murine leukemia virus and pseudotypes with greater sensitivity and more rapidly than previously possible. To achieve this, we encapsulated a sensitive reporter enzyme, luciferase, directly into fully infectious, intact viral particles."
"The enzyme is specifically targeted to the viral lumen, as a C-terminal fusion on the viral envelope protein. Only when the incorporated luciferase is released from the viral lumen and gains access to its...
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Source: Blood Weekly (2004-07-22)
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