Coronavirus

Sensitive/specific EIA detects nucleocapsid antigen in sera from SARS patients

Published in Blood Weekly, August 26th, 2004

A sensitive and specific enzyme immunoassay detects nucleocapsid antigen in sera from SARS patients.

"A rapid antigen test for the diagnosis of severe acute respiratory syndrome (SARS) is essential for control of this disease at the point of management.

"The nucleocapsid (N) protein of SARS-associated coronavirus (SARS-CoV) is abundantly expressed in infected-cell culture filtrate as demonstrable by Western blotting using convalescent-phase sera from patients with SARS," scientists in China report.

"We used monoclonal antibodies specifically directed against N protein to establish a sensitive antigen capture sandwich enzyme-linked...

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Source: Blood Weekly (2004-08-26)

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