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Biotechnology
microRNAs quantitated using a modified Invader assay
September 9th, 2004
microRNAs were quantitated using a modified Invader assay. According to a study from the United States, "The short lengths of microRNAs (miRNAs) present a significant challenge for detection and quantitation using conventional methods for RNA analysis. "To address this problem, we developed a quantitative, sensitive, and rapid miRNA assay based on our previously described messenger RNA Invader assay." "This assay was used successfully in the analysis of several miRNAs, using as little as 50-100 ng of total cellular RNA or as few as 1,000 lysed cells. Its specificity," said H.T. Allawi and coworkers, "allowed for discrimination...
Source: Blood Weekly (2004-09-09)
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